Hotspot

tilS

Genome · 36607-37584 · 978 bp

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Pi 0.268

MarkerSeek score 81.20

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 978
variable_sites: 527
pi: 0.268
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	GCAATTCGTGATGCTGATCG	CGCGCACAACATCAGATTTT	50.0	45.0	58.0	58.0	1992 bp	0.166667	46.3
tilS_p2	GCAATTCGTGATGCTGATCG	CGCATAGGCATTTTAGCTGC	50.0	50.0	58.0	58.0	1746 bp	0.166667	46.2
tilS_p3	GCAATTCGTGATGCTGATCG	CCTTTCATACGACCTCGGTG	50.0	55.0	58.0	58.1	1419 bp	0.166667	45.9
tilS_p4	GCAATTCGTGATGCTGATCG	CTTTCATACGACCTCGGTGG	50.0	55.0	58.0	58.1	1418 bp	0.166667	45.9
tilS_p5	GATCGTGTTGCGGGATTACT	CGCGCACAACATCAGATTTT	50.0	45.0	58.1	58.0	1977 bp	0.166667	45.9