Hotspot

psbD

Genome · 10930-11988 · 1059 bp

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Pi 0.0891

MarkerSeek score 78.80

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: Genome
length_bp: 1059
variable_sites: 211
pi: 0.0891
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	CCTTCACTTCCACAAGCCTT	CTGACCAGATGAATGCACGA	50.0	50.0	58.0	58.0	2375 bp	0.166667	46.4
psbD_p2	CCTTCACTTCCACAAGCCTT	GTTACCAGACCACCAAGCAA	50.0	50.0	58.0	58.0	1690 bp	0.166667	46.4
psbD_p3	CCTTCACTTCCACAAGCCTT	TTCTGGAACGAAGTGTGCAA	50.0	45.0	58.0	58.0	1807 bp	0.166667	46.3
psbD_p4	CCTTCACTTCCACAAGCCTT	TTTCTGGAACGAAGTGTGCA	50.0	45.0	58.0	58.0	1808 bp	0.166667	46.3
psbD_p5	AGGGTGAATATGCTGAAGCG	CTGACCAGATGAATGCACGA	50.0	50.0	58.0	58.0	2539 bp	0.166667	46.2