Hotspot

petB

Genome · 29754-30401 · 648 bp

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Pi 0.091

MarkerSeek score 79.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: Genome
length_bp: 648
variable_sites: 131
pi: 0.091
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGGTGCTATCTCTGCAATGC	ACACGATACCATGGTAACGC	50.0	50.0	58.0	58.1	2059-2110 bp	0.333333	62.8
petB_p2	AGGTGCTATCTCTGCAATGC	TACAACACTTGCCACTGGAC	50.0	50.0	58.0	58.0	1871-1883 bp	0.333333	61.9
petB_p3	AGGTGCTATCTCTGCAATGC	GAGACAAGACAGTAAGGCGG	50.0	55.0	58.0	58.0	1451 bp	0.166667	46.4
petB_p4	AGGTGCTATCTCTGCAATGC	TGCAAAAGGGTTAGCTGGTT	50.0	45.0	58.0	57.9	1697 bp	0.166667	45.7
petB_p5	AGGTGCTATCTCTGCAATGC	TTGCAAAAGGGTTAGCTGGT	50.0	45.0	58.0	57.9	1698 bp	0.166667	45.7