Hotspot

petA

Genome · 14157-15071 · 915 bp

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Pi 0.193

MarkerSeek score 79.50

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: Genome
length_bp: 915
variable_sites: 362
pi: 0.193
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AAGGATACGGGATAGACGGG	ACGACCTGGACGTTTTGAAA	55.0	45.0	58.1	58.0	2023 bp	0.166667	45.8
petA_p2	CCTACGCTGGTTCAAATCCA	ACGACCTGGACGTTTTGAAA	50.0	45.0	57.9	58.0	2220 bp	0.166667	45.6
petA_p3	TGGTTAAAGGAGGCGGATTG	ACGACCTGGACGTTTTGAAA	50.0	45.0	57.9	58.0	2257 bp	0.166667	45.5
petA_p4	GGTTAAAGGAGGCGGATTGT	ACGACCTGGACGTTTTGAAA	50.0	45.0	57.9	58.0	2256 bp	0.166667	45.5
petA_p5	GTGGTTAAAGGAGGCGGATT	ACGACCTGGACGTTTTGAAA	50.0	45.0	57.9	58.0	2258 bp	0.166667	45.5