Hotspot

minD

Genome · 35700-36617 · 918 bp

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Pi 0.181

MarkerSeek score 79.80

Primer available yes

Label minD

Feature Metadata

label_name: minD
feature_type: gene
region: Genome
length_bp: 918
variable_sites: 343
pi: 0.181
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
minD_p1	GGTCTGTTGGGGAAGGTTTT	ATTGACGACTTTCAGCCTCC	50.0	50.0	57.9	57.9	1521 bp	0.166667	45.2
minD_p2	AAGCAACCGAATTAGCTCGT	ATTGACGACTTTCAGCCTCC	45.0	50.0	57.9	57.9	1284 bp	0.166667	45.1
minD_p3	AGCAACCGAATTAGCTCGTT	ATTGACGACTTTCAGCCTCC	45.0	50.0	57.9	57.9	1283 bp	0.166667	45.1
minD_p4	CCGGAATTGTTCTTCTCGGT	ATTGACGACTTTCAGCCTCC	50.0	50.0	57.9	57.9	1683 bp	0.166667	45.1
minD_p5	CCGTTACGGTTTTCCTGGAA	ATTGACGACTTTCAGCCTCC	50.0	50.0	58.1	57.9	1463 bp	0.166667	44.9