Hotspot

ftsH

Genome · 15241-16515 · 1275 bp

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Pi 0.228

MarkerSeek score 81.40

Primer available yes

Label ftsH

Feature Metadata

label_name: ftsH
feature_type: gene
region: Genome
length_bp: 1275
variable_sites: 583
pi: 0.228
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH_p1	TTGCTTCACGTGGTTCTTCA	AAAGCGCTCGATGGTTACAT	45.0	45.0	58.0	58.0	1937-1938 bp	0.333333	61.3
ftsH_p2	TCTTCGTAACGGGCAAGAAG	AAAGCGCTCGATGGTTACAT	50.0	45.0	57.9	58.0	1784-1787 bp	0.333333	60.9
ftsH_p3	GTAACGGGCAAGAAGGGATT	AAAGCGCTCGATGGTTACAT	50.0	45.0	57.9	58.0	1779-1782 bp	0.333333	60.6
ftsH_p4	TAAACTTGTCCACGACCACG	AAAGCGCTCGATGGTTACAT	50.0	45.0	58.1	58.0	2358-2359 bp	0.333333	60.3
ftsH_p5	TTGCTTCACGTGGTTCTTCA	AAAAGCGCTCGATGGTTACA	45.0	45.0	58.0	58.2	1938-1939 bp	0.333333	59.9