Hotspot

atpI

Genome · 54581-55294 · 714 bp

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Pi 0.127

MarkerSeek score 79.30

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: Genome
length_bp: 714
variable_sites: 205
pi: 0.127
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	ACAAAACCTCAAGCAGCTCA	ACCAGGTCCAATGGAACCTA	45.0	50.0	57.9	57.9	1451-1457 bp	0.333333	60.4
atpI_p2	ACAAAACCTCAAGCAGCTCA	TACCAGGTCCAATGGAACCT	45.0	50.0	57.9	57.9	1452-1458 bp	0.333333	60.4
atpI_p3	ACAAAACCTCAAGCAGCTCA	TAACCTGCAGCAGTTCCTTG	45.0	50.0	57.9	58.1	1477-1483 bp	0.333333	60.0
atpI_p4	ACAAAACCTCAAGCAGCTCA	GTAACCTGCAGCAGTTCCTT	45.0	50.0	57.9	58.1	1478 bp	0.166667	45.4
atpI_p5	CAACGTTGGCTAGGAGGTTT	ACCAGGTCCAATGGAACCTA	50.0	50.0	58.1	57.9	1385 bp	0.166667	45.4