Hotspot

atpB

Genome · 7845-9287 · 1443 bp

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Pi 0.113

MarkerSeek score 79.20

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: Genome
length_bp: 1443
variable_sites: 350
pi: 0.113
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	AATGATCCAAGCCGTAACCC	ACTTGGCAACGTGATTCTGT	50.0	45.0	57.9	58.0	2237 bp	0.166667	46.0
atpB_p2	AATGATCCAAGCCGTAACCC	AGTCACTTGGCAACGTGATT	50.0	45.0	57.9	58.0	2241 bp	0.166667	46.0
atpB_p3	AATGATCCAAGCCGTAACCC	GAATACGTAAACGCGTCCCT	50.0	50.0	57.9	58.1	2173 bp	0.166667	45.6
atpB_p4	AATGATCCAAGCCGTAACCC	AACGTAACGGCCCAATTTCT	50.0	45.0	57.9	58.1	1770 bp	0.166667	45.4
atpB_p5	AATGATCCAAGCCGTAACCC	CCAGACTCAGCCTGGTTTAC	50.0	55.0	57.9	57.9	1873 bp	0.166667	45.4