Hotspot

trnS(gcu)-trnL(uag)

Genome · 64179-64234 · 56 bp

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Pi 0.324
MarkerSeek score 78.90
Primer available yes
Label trnS(gcu)-trnL(uag)

Feature Metadata

label_name
trnS(gcu)-trnL(uag)
feature_type
igs
region
Genome
length_bp
56
variable_sites
35
pi
0.324
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(gcu)-trnL(uag)_p1 TTCCTTGGCCTTTTGTCGAA CAGGGGTTGGATTTGAACCA 45.0 50.0 57.9 58.0 901 bp 0.166667 46.1
trnS(gcu)-trnL(uag)_p2 TTTCCTTGGCCTTTTGTCGA CAGGGGTTGGATTTGAACCA 45.0 50.0 57.9 58.0 902 bp 0.166667 46.1
trnS(gcu)-trnL(uag)_p3 TTTATCGTCTGCACCTGTGG CAGGGGTTGGATTTGAACCA 50.0 50.0 57.9 58.0 1078 bp 0.166667 45.9
trnS(gcu)-trnL(uag)_p4 TTCCTTGGCCTTTTGTCGAA CATTTTGATGCCTCCGGAGA 45.0 50.0 57.9 58.0 488 bp 0.166667 45.8
trnS(gcu)-trnL(uag)_p5 TTTCCTTGGCCTTTTGTCGA CATTTTGATGCCTCCGGAGA 45.0 50.0 57.9 58.0 489 bp 0.166667 45.8