primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score chlB-psbI_p1 chlB-psbI 1 CAAGTGGACTCCAGATGCTC ATTCTGGAGATGGGTGGTGA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.050206 6824 7615 1563-1563 0.166667 46.3 chlB-psbI_p2 chlB-psbI 2 CAAGTGGACTCCAGATGCTC TCTGGAGATGGGTGGTGAAT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.050206 6824 7615 1561-1561 0.166667 46.3 chlB-psbI_p3 chlB-psbI 3 ATGTTGTTTGTGCGGGTACT ATTCTGGAGATGGGTGGTGA 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.053191 6824 7615 1957-1957 0.166667 46.2 chlB-psbI_p4 chlB-psbI 4 ATGTTGTTTGTGCGGGTACT TCTGGAGATGGGTGGTGAAT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.053191 6824 7615 1955-1955 0.166667 46.2 chlB-psbI_p5 chlB-psbI 5 CAAGTGGACTCCAGATGCTC GGGTTACGGCTTGGATCATT 20 20 55.0 50.0 58.0 57.9 0.0 0.0 0.074137 6824 7615 1044-1044 0.166667 46.1 atpB_p1 atpB 1 AATGATCCAAGCCGTAACCC ACTTGGCAACGTGATTCTGT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.085464 7845 9287 2237-2237 0.166667 46.0 atpB_p2 atpB 2 AATGATCCAAGCCGTAACCC AGTCACTTGGCAACGTGATT 20 20 50.0 45.0 57.9 58.0 0.0 24.8 0.085464 7845 9287 2241-2241 0.166667 46.0 atpB_p3 atpB 3 AATGATCCAAGCCGTAACCC GAATACGTAAACGCGTCCCT 20 20 50.0 50.0 57.9 58.1 0.0 14.4 0.139466 7845 9287 2173-2173 0.166667 45.6 atpB_p4 atpB 4 AATGATCCAAGCCGTAACCC AACGTAACGGCCCAATTTCT 20 20 50.0 45.0 57.9 58.1 0.0 0.0 0.152537 7845 9287 1770-1770 0.166667 45.4 atpB_p5 atpB 5 AATGATCCAAGCCGTAACCC CCAGACTCAGCCTGGTTTAC 20 20 50.0 55.0 57.9 57.9 0.0 0.0 0.153387 7845 9287 1873-1873 0.166667 45.4 psbD_p1 psbD 1 CCTTCACTTCCACAAGCCTT CTGACCAGATGAATGCACGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.027453 10930 11988 2375-2375 0.166667 46.4 psbD_p2 psbD 2 CCTTCACTTCCACAAGCCTT GTTACCAGACCACCAAGCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.037458 10930 11988 1690-1690 0.166667 46.4 psbD_p3 psbD 3 CCTTCACTTCCACAAGCCTT TTCTGGAACGAAGTGTGCAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.044376 10930 11988 1807-1807 0.166667 46.3 psbD_p4 psbD 4 CCTTCACTTCCACAAGCCTT TTTCTGGAACGAAGTGTGCA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.044376 10930 11988 1808-1808 0.166667 46.3 psbD_p5 psbD 5 AGGGTGAATATGCTGAAGCG CTGACCAGATGAATGCACGA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055294 10930 11988 2539-2539 0.166667 46.2 petA_p1 petA 1 AAGGATACGGGATAGACGGG ACGACCTGGACGTTTTGAAA 20 20 55.0 45.0 58.1 58.0 0.0 18.0 0.106393 14157 15071 2023-2023 0.166667 45.8 petA_p2 petA 2 CCTACGCTGGTTCAAATCCA ACGACCTGGACGTTTTGAAA 20 20 50.0 45.0 57.9 58.0 0.0 18.0 0.134953 14157 15071 2220-2220 0.166667 45.6 petA_p3 petA 3 TGGTTAAAGGAGGCGGATTG ACGACCTGGACGTTTTGAAA 20 20 50.0 45.0 57.9 58.0 0.0 18.0 0.149787 14157 15071 2257-2257 0.166667 45.5 petA_p4 petA 4 GGTTAAAGGAGGCGGATTGT ACGACCTGGACGTTTTGAAA 20 20 50.0 45.0 57.9 58.0 0.0 18.0 0.151281 14157 15071 2256-2256 0.166667 45.5 petA_p5 petA 5 GTGGTTAAAGGAGGCGGATT ACGACCTGGACGTTTTGAAA 20 20 50.0 45.0 57.9 58.0 0.0 18.0 0.151281 14157 15071 2258-2258 0.166667 45.5 ftsH_p1 ftsH 1 TTGCTTCACGTGGTTCTTCA AAAGCGCTCGATGGTTACAT 20 20 45.0 45.0 58.0 58.0 17.0 12.1 0.055434 15241 16515 1937-1938 0.333333 61.3 ftsH_p2 ftsH 2 TCTTCGTAACGGGCAAGAAG AAAGCGCTCGATGGTTACAT 20 20 50.0 45.0 57.9 58.0 0.0 12.1 0.098723 15241 16515 1784-1787 0.333333 60.9 ftsH_p3 ftsH 3 GTAACGGGCAAGAAGGGATT AAAGCGCTCGATGGTTACAT 20 20 50.0 45.0 57.9 58.0 0.0 12.1 0.148453 15241 16515 1779-1782 0.333333 60.6 ftsH_p4 ftsH 4 TAAACTTGTCCACGACCACG AAAGCGCTCGATGGTTACAT 20 20 50.0 45.0 58.1 58.0 0.0 12.1 0.168662 15241 16515 2358-2359 0.333333 60.3 ftsH_p5 ftsH 5 TTGCTTCACGTGGTTCTTCA AAAAGCGCTCGATGGTTACA 20 20 45.0 45.0 58.0 58.2 17.0 12.1 0.227924 15241 16515 1938-1939 0.333333 59.9 ycf12-rbcL_p1 ycf12-rbcL 1 AATATAGGGACGGTGAGCGA CACCACGTAAGCACTCGTAA 20 20 50.0 50.0 58.0 57.9 12.3 0.0 0.063566 22978 23081 920-920 0.166667 46.2 ycf12-rbcL_p2 ycf12-rbcL 2 AATATAGGGACGGTGAGCGA CCACGTAAGCACTCGTAACA 20 20 50.0 50.0 58.0 57.9 12.3 0.0 0.063566 22978 23081 918-918 0.166667 46.2 ycf12-rbcL_p3 ycf12-rbcL 3 AATATAGGGACGGTGAGCGA AACAAGCACGACCGTAGTTT 20 20 50.0 45.0 58.0 58.1 12.3 0.0 0.064847 22978 23081 902-902 0.166667 46.1 ycf12-rbcL_p4 ycf12-rbcL 4 AATATAGGGACGGTGAGCGA ACAAGCACGACCGTAGTTTT 20 20 50.0 45.0 58.0 58.1 12.3 0.0 0.064847 22978 23081 901-901 0.166667 46.1 ycf12-rbcL_p5 ycf12-rbcL 5 AATATAGGGACGGTGAGCGA GTAACAAGCACGACCGTAGT 20 20 50.0 50.0 58.0 57.9 12.3 0.0 0.064973 22978 23081 904-904 0.166667 46.1 petB_p1 petB 1 AGGTGCTATCTCTGCAATGC ACACGATACCATGGTAACGC 20 20 50.0 50.0 58.0 58.1 0.0 39.0 0.104880 29754 30401 2059-2110 0.333333 62.8 petB_p2 petB 2 AGGTGCTATCTCTGCAATGC TACAACACTTGCCACTGGAC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.073654 29754 30401 1871-1883 0.333333 61.9 petB_p3 petB 3 AGGTGCTATCTCTGCAATGC GAGACAAGACAGTAAGGCGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.037778 29754 30401 1451-1451 0.166667 46.4 petB_p4 petB 4 AGGTGCTATCTCTGCAATGC TGCAAAAGGGTTAGCTGGTT 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.116101 29754 30401 1697-1697 0.166667 45.7 petB_p5 petB 5 AGGTGCTATCTCTGCAATGC TTGCAAAAGGGTTAGCTGGT 20 20 50.0 45.0 58.0 57.9 0.0 0.0 0.116101 29754 30401 1698-1698 0.166667 45.7 psbB_p1 psbB 1 CCGCCTTACTGTCTTGTCTC CCTTACCACTTGGCTACGAC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.008301 31101 32639 2744-2744 0.166667 46.6 psbB_p2 psbB 2 CCGCCTTACTGTCTTGTCTC CTTACCACTTGGCTACGACC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.008301 31101 32639 2743-2743 0.166667 46.6 psbB_p3 psbB 3 CCGCCTTACTGTCTTGTCTC ACCAGGGGCTACTTTACCAT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.021071 31101 32639 2447-2447 0.166667 46.5 psbB_p4 psbB 4 GTCCAGTGGCAAGTGTTGTA CCTTACCACTTGGCTACGAC 20 20 50.0 55.0 58.0 58.0 3.5 0.0 0.044177 31101 32639 2312-2312 0.166667 46.3 psbB_p5 psbB 5 GTCCAGTGGCAAGTGTTGTA CTTACCACTTGGCTACGACC 20 20 50.0 55.0 58.0 58.0 3.5 0.0 0.044177 31101 32639 2311-2311 0.166667 46.3 minD_p1 minD 1 GGTCTGTTGGGGAAGGTTTT ATTGACGACTTTCAGCCTCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.182745 35700 36617 1521-1521 0.166667 45.2 minD_p2 minD 2 AAGCAACCGAATTAGCTCGT ATTGACGACTTTCAGCCTCC 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.195367 35700 36617 1284-1284 0.166667 45.1 minD_p3 minD 3 AGCAACCGAATTAGCTCGTT ATTGACGACTTTCAGCCTCC 20 20 45.0 50.0 57.9 57.9 0.0 0.0 0.195367 35700 36617 1283-1283 0.166667 45.1 minD_p4 minD 4 CCGGAATTGTTCTTCTCGGT ATTGACGACTTTCAGCCTCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.195367 35700 36617 1683-1683 0.166667 45.1 minD_p5 minD 5 CCGTTACGGTTTTCCTGGAA ATTGACGACTTTCAGCCTCC 20 20 50.0 50.0 58.1 57.9 16.5 0.0 0.219014 35700 36617 1463-1463 0.166667 44.9 tilS_p1 tilS 1 GCAATTCGTGATGCTGATCG CGCGCACAACATCAGATTTT 20 20 50.0 45.0 58.0 58.0 0.0 8.7 0.046879 36607 37584 1992-1992 0.166667 46.3 tilS_p2 tilS 2 GCAATTCGTGATGCTGATCG CGCATAGGCATTTTAGCTGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.056745 36607 37584 1746-1746 0.166667 46.2 tilS_p3 tilS 3 GCAATTCGTGATGCTGATCG CCTTTCATACGACCTCGGTG 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.092071 36607 37584 1419-1419 0.166667 45.9 tilS_p4 tilS 4 GCAATTCGTGATGCTGATCG CTTTCATACGACCTCGGTGG 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.092071 36607 37584 1418-1418 0.166667 45.9 tilS_p5 tilS 5 GATCGTGTTGCGGGATTACT CGCGCACAACATCAGATTTT 20 20 50.0 45.0 58.1 58.0 0.0 8.7 0.096306 36607 37584 1977-1977 0.166667 45.9 rpoB_p1 rpoB 1 AATGGGCGTTAGCTCTAAGC AATGAAGTACTTCGACGGGC 20 20 50.0 50.0 58.0 58.0 0.0 26.4 0.051044 47090 47209 771-771 0.166667 46.3 rpoB_p2 rpoB 2 AATGGGCGTTAGCTCTAAGC GGCCTGAACGCCCTAATAAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.092075 47090 47209 1097-1097 0.166667 45.9 rpoB_p3 rpoB 3 AATGGGCGTTAGCTCTAAGC AATTGGGTTTGTTTGGTGCG 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.098328 47090 47209 736-736 0.166667 45.9 rpoB_p4 rpoB 4 AATGGGCGTTAGCTCTAAGC CCCATTAAGGCCCTGTTACC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.112438 47090 47209 1224-1224 0.166667 45.8 rpoB_p5 rpoB 5 ATCTTACCGCAACAAGGGAC AATGAAGTACTTCGACGGGC 20 20 50.0 50.0 57.9 58.0 0.0 26.4 0.115395 47090 47209 887-887 0.166667 45.7 rpoC2_p1 rpoC2 1 GTGATCAGATGGCGGTTCAT AATGGGGCCATTTTAGGGTG 20 20 50.0 50.0 58.0 58.1 0.0 4.4 0.162748 51427 53736 2901-2901 0.166667 45.4 rpoC2_p2 rpoC2 2 TTGATGGTGATCAGATGGCG AATGGGGCCATTTTAGGGTG 20 20 50.0 50.0 58.0 58.1 0.0 4.4 0.164121 51427 53736 2907-2907 0.166667 45.4 rpoC2_p3 rpoC2 3 ACTTTACAGGGTGGTCAGGA TGAGCTGCTTGAGGTTTTGT 20 20 50.0 45.0 57.9 57.9 0.0 2.5 0.169416 51427 53736 2775-2775 0.166667 45.3 rpoC2_p4 rpoC2 4 TGTTTCTGGCCGTGCTATTT AATGGGGCCATTTTAGGGTG 20 20 45.0 50.0 58.1 58.1 0.0 4.4 0.217502 51427 53736 2966-2966 0.166667 44.9 rpoC2_p5 rpoC2 5 TTGTTTCTGGCCGTGCTATT AATGGGGCCATTTTAGGGTG 20 20 45.0 50.0 58.1 58.1 0.0 4.4 0.217502 51427 53736 2967-2967 0.166667 44.9 atpI_p1 atpI 1 ACAAAACCTCAAGCAGCTCA ACCAGGTCCAATGGAACCTA 20 20 45.0 50.0 57.9 57.9 0.0 14.7 0.110884 54581 55294 1451-1457 0.333333 60.4 atpI_p2 atpI 2 ACAAAACCTCAAGCAGCTCA TACCAGGTCCAATGGAACCT 20 20 45.0 50.0 57.9 57.9 0.0 14.7 0.110884 54581 55294 1452-1458 0.333333 60.4 atpI_p3 atpI 3 ACAAAACCTCAAGCAGCTCA TAACCTGCAGCAGTTCCTTG 20 20 45.0 50.0 57.9 58.1 0.0 21.3 0.160918 54581 55294 1477-1483 0.333333 60.0 atpI_p4 atpI 4 ACAAAACCTCAAGCAGCTCA GTAACCTGCAGCAGTTCCTT 20 20 45.0 50.0 57.9 58.1 0.0 21.3 0.159577 54581 55294 1478-1478 0.166667 45.4 atpI_p5 atpI 5 CAACGTTGGCTAGGAGGTTT ACCAGGTCCAATGGAACCTA 20 20 50.0 50.0 58.1 57.9 20.7 14.7 0.161537 54581 55294 1385-1385 0.166667 45.4 trnS(gcu)-trnL(uag)_p1 trnS(gcu)-trnL(uag) 1 TTCCTTGGCCTTTTGTCGAA CAGGGGTTGGATTTGAACCA 20 20 45.0 50.0 57.9 58.0 3.3 0.0 0.068293 64179 64234 901-901 0.166667 46.1 trnS(gcu)-trnL(uag)_p2 trnS(gcu)-trnL(uag) 2 TTTCCTTGGCCTTTTGTCGA CAGGGGTTGGATTTGAACCA 20 20 45.0 50.0 57.9 58.0 3.3 0.0 0.068293 64179 64234 902-902 0.166667 46.1 trnS(gcu)-trnL(uag)_p3 trnS(gcu)-trnL(uag) 3 TTTATCGTCTGCACCTGTGG CAGGGGTTGGATTTGAACCA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.101549 64179 64234 1078-1078 0.166667 45.9 trnS(gcu)-trnL(uag)_p4 trnS(gcu)-trnL(uag) 4 TTCCTTGGCCTTTTGTCGAA CATTTTGATGCCTCCGGAGA 20 20 45.0 50.0 57.9 58.0 3.3 33.4 0.102736 64179 64234 488-488 0.166667 45.8 trnS(gcu)-trnL(uag)_p5 trnS(gcu)-trnL(uag) 5 TTTCCTTGGCCTTTTGTCGA CATTTTGATGCCTCCGGAGA 20 20 45.0 50.0 57.9 58.0 3.3 33.4 0.102736 64179 64234 489-489 0.166667 45.8