Hotspot

ycf1

SSC · 123890-129028 · 5139 bp

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Pi 0.0286

MarkerSeek score 61.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5139
variable_sites: 147
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTCTACGACGTCTGGGAGAT	ACCTTGACCAATACGTATGCC	55.0	47.6	58.0	58.1	814 bp	0.5	51.0
ycf1_p2	CTCTACGACGTCTGGGAGAT	CCTTGACCAATACGTATGCCA	55.0	47.6	58.0	58.1	813 bp	0.5	51.0
ycf1_p3	TTCTCTACGACGTCTGGGAG	ACCTTGACCAATACGTATGCC	55.0	47.6	58.3	58.1	816 bp	0.5	49.2
ycf1_p4	TTCTCTACGACGTCTGGGAG	CCTTGACCAATACGTATGCCA	55.0	47.6	58.3	58.1	815 bp	0.5	49.2
ycf1_p5	ACGACGTCTGGGAGATAGAA	ACCTTGACCAATACGTATGCC	50.0	47.6	57.6	58.1	810 bp	0.5	48.0