Hotspot

ndhD

SSC · 115448-116950 · 1503 bp

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Pi 0.0226

MarkerSeek score 58.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 34
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGCTTTCTATCTCATTAATGAAAA	ACCGAGAAAAACCCGTACTC	25.0	50.0	52.8	57.6	1605 bp	1.0	41.7
ndhD_p2	TTGCTTTCTATCTCATTAATGAAA	ACCGAGAAAAACCCGTACTC	25.0	50.0	52.8	57.6	1606 bp	1.0	41.7
ndhD_p3	TTTGCTTTCTATCTCATTAATGAA	ACCGAGAAAAACCCGTACTC	25.0	50.0	52.8	57.6	1607 bp	1.0	41.7
ndhD_p4	TGCTTTCTATCTCATTAATGAAAA	CCGAGAAAAACCCGTACTCG	25.0	55.0	52.8	58.7	1604 bp	1.0	41.7
ndhD_p5	TTGCTTTCTATCTCATTAATGAAA	CCGAGAAAAACCCGTACTCG	25.0	55.0	52.8	58.7	1605 bp	1.0	41.7