Hotspot

atpA

LSC · 10207-11730 · 1524 bp

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Pi 0.0184

MarkerSeek score 58.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 28
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGATCTTGTACTAAGAAATGATCT	TTTTCCTTTGTTTCCGAAAAA	29.2	28.6	53.3	52.9	1614 bp	0.5	20.0
atpA_p2	TGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAA	29.2	28.6	53.3	52.9	1615 bp	0.5	20.0
atpA_p3	TGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAAA	29.2	27.3	53.3	53.8	1615 bp	0.5	20.0
atpA_p4	ATGATCTTGTACTAAGAAATGATCT	TTTTCCTTTGTTTCCGAAAAA	28.0	28.6	53.8	52.9	1615 bp	0.5	20.0
atpA_p5	ATGATCTTGTACTAAGAAATGATCT	TTTTTCCTTTGTTTCCGAAAA	28.0	28.6	53.8	52.9	1616 bp	0.5	20.0