Hotspot

rrn5-rrn4.5

IRa · 130440-130660 · 221 bp

Back to Chlorophytum

Pi 0.0505

MarkerSeek score 79.20

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 221
variable_sites: 11
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	335-338 bp	1.0	82.7
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	333-336 bp	1.0	82.7
rrn5-rrn4.5_p3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	331-334 bp	1.0	82.7
rrn5-rrn4.5_p4	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	333-336 bp	1.0	82.6
rrn5-rrn4.5_p5	TCCCCTACAGTATCGTCACC	CAGCTGAGGCATCCTAACAG	55.0	55.0	57.9	58.0	343-346 bp	1.0	82.4