Hotspot

ndhF-rpl32

SSC · 112371-113301 · 931 bp

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Pi 0.0431

MarkerSeek score 75.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 931
variable_sites: 38
pi: 0.0431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAATTGGTCGTGTGAAC	AGGACTAGACCGAGTGTACAG	50.0	52.4	58.0	58.0	1772-1788 bp	1.0	76.2
ndhF-rpl32_p2	CTGCAATTGGTCGTGTGAAC	GGACTAGACCGAGTGTACAGA	50.0	52.4	58.0	58.0	1771-1787 bp	1.0	76.2
ndhF-rpl32_p3	GCGCAGATTTAGCAATTGCA	AGGACTAGACCGAGTGTACAG	45.0	52.4	58.1	58.0	1985-2001 bp	1.0	75.4
ndhF-rpl32_p4	TGCGCAGATTTAGCAATTGC	AGGACTAGACCGAGTGTACAG	45.0	52.4	58.1	58.0	1986-2002 bp	1.0	75.4
ndhF-rpl32_p5	GCGCAGATTTAGCAATTGCA	GGACTAGACCGAGTGTACAGA	45.0	52.4	58.1	58.0	1984-2000 bp	1.0	75.3