Hotspot

ndhC-trnV-UAC

LSC · 50225-51819 · 1595 bp

Back to Chlorophytum

Pi 0.0382
MarkerSeek score 71.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1595
variable_sites
59
pi
0.0382
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGTATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.9 57.8 1623-1650 bp 1.0 67.4
ndhC-trnV-UAC_p2 TCGTATTCGTGAAGCAGAAAC AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.7 57.8 1624-1651 bp 1.0 65.2
ndhC-trnV-UAC_p3 TCGTATTCGTGAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 40.9 50.0 58.4 57.8 1624-1651 bp 1.0 65.0
ndhC-trnV-UAC_p4 CGTATTCGTGAAGCAGAAACA GAAGGTCTACGGTTCGAGTC 42.9 55.0 56.9 57.5 1622-1649 bp 1.0 64.7
ndhC-trnV-UAC_p5 CGTATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 42.9 55.0 56.9 58.7 1611-1638 bp 1.0 63.6