Hotspot

ycf3-trnS-GGA

LSC · 44170-44717 · 548 bp

Back to Chlorophytum

Pi 0.031

MarkerSeek score 68.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 17
pi: 0.031
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CCATTTATCCGGGATCTAGGC	ACGGAAAGAGAGGGATTCGA	52.4	50.0	57.7	58.2	660 bp	1.0	70.7
ycf3-trnS-GGA_p2	CCATTTATCCGGGATCTAGGC	TTCCAATGCTACGCCTTCAA	52.4	45.0	57.7	57.8	609 bp	1.0	70.5
ycf3-trnS-GGA_p3	CCATTTATCCGGGATCTAGGC	AGTTCCAATGCTACGCCTTC	52.4	50.0	57.7	58.3	611 bp	1.0	70.0
ycf3-trnS-GGA_p4	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	733 bp	1.0	68.0
ycf3-trnS-GGA_p5	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	734 bp	1.0	68.0