Hotspot

rps9

Genome · 39248-39844 · 597 bp

Back to Chloroparvula

Pi 0.491

MarkerSeek score 82.10

Primer available yes

Label rps9

Feature Metadata

label_name: rps9
feature_type: gene
region: Genome
length_bp: 597
variable_sites: 263
pi: 0.491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps9_p1	TCGCGAAGGTGTATTGACTG	ACAGCGCGATTTGTAGACAT	50.0	45.0	58.0	58.0	1810 bp	0.5	59.8
rps9_p2	TCGCGAAGGTGTATTGACTG	TTTGCAAGTAACCACGTCGA	50.0	45.0	58.0	58.1	1243 bp	0.5	59.4
rps9_p3	ACAGTTCAACCAGTGGCATT	ACAGCGCGATTTGTAGACAT	45.0	45.0	57.9	58.0	1548 bp	0.5	59.3
rps9_p4	TTCAACCAGTGGCATTTCCA	ACAGCGCGATTTGTAGACAT	45.0	45.0	57.9	58.0	1544 bp	0.5	59.3
rps9_p5	TCGCGAAGGTGTATTGACTG	TGTTGCTTGACGATCCTCAG	50.0	50.0	58.0	57.9	1687 bp	0.5	59.2