Hotspot

rps8

Genome · 37180-37608 · 429 bp

Back to Chloroparvula

Pi 0.507

MarkerSeek score 79.90

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: Genome
length_bp: 429
variable_sites: 206
pi: 0.507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	CTCGTGAGCTTCGTGATCAA	ATCACGCGACATGAATCACA	50.0	45.0	58.0	58.0	1509 bp	0.5	59.8
rps8_p2	CTCGTGAGCTTCGTGATCAA	CCATTGGGGTGCTAGGAATC	50.0	55.0	58.0	58.0	1157 bp	0.5	59.8
rps8_p3	TTAACCTTGCTTTGCCTCGT	ATCACGCGACATGAATCACA	45.0	45.0	58.0	58.0	1104 bp	0.5	59.7
rps8_p4	CTCGTGAGCTTCGTGATCAA	CTTAAAACCACATGCGCCTG	50.0	50.0	58.0	58.0	1753 bp	0.5	59.7
rps8_p5	TTAACCTTGCTTTGCCTCGT	CCATTGGGGTGCTAGGAATC	45.0	55.0	58.0	58.0	752 bp	0.5	59.7