Hotspot

rpl19

Genome · 33931-34296 · 366 bp

Back to Chloroparvula

Pi 0.537

MarkerSeek score 77.10

Primer available yes

Label rpl19

Feature Metadata

label_name: rpl19
feature_type: gene
region: Genome
length_bp: 366
variable_sites: 190
pi: 0.537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl19_p1	GTTATGCCAGGTGATCGTGT	ATCTACACGGCGTCCTTTTC	50.0	50.0	58.0	58.0	951 bp	0.5	59.8
rpl19_p2	GTTATGCCAGGTGATCGTGT	AGACCTTGAGGCAAAAACGT	50.0	45.0	58.0	57.9	791 bp	0.5	59.4
rpl19_p3	GTTATGCCAGGTGATCGTGT	TCCACAGCATTGTCAACCAA	50.0	45.0	58.0	57.9	859 bp	0.5	59.3
rpl19_p4	GTTATGCCAGGTGATCGTGT	TTCCACAGCATTGTCAACCA	50.0	45.0	58.0	57.9	860 bp	0.5	59.3
rpl19_p5	TGCCAGGTGATCGTGTAAAG	ATCTACACGGCGTCCTTTTC	50.0	50.0	57.9	58.0	947 bp	0.5	59.2