Hotspot

psbD

Genome · 5318-6376 · 1059 bp

Back to Chloroparvula

Pi 0.448

MarkerSeek score 79.70

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: Genome
length_bp: 1059
variable_sites: 470
pi: 0.448
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	GCCGAGGCATACTAATAGGC	ACCACCAAAGCCAAGTACAG	55.0	50.0	58.0	58.0	1761 bp	0.5	59.8
psbD_p2	GCCGAGGCATACTAATAGGC	GGTACAAAATGCGCAACCTC	55.0	50.0	58.0	58.0	1604 bp	0.5	59.7
psbD_p3	GGTACATGAGCTGGGTTCAG	ACCACCAAAGCCAAGTACAG	55.0	50.0	58.0	58.0	2113 bp	0.5	59.6
psbD_p4	GGTACATGAGCTGGGTTCAG	GGTACAAAATGCGCAACCTC	55.0	50.0	58.0	58.0	1956 bp	0.5	59.6
psbD_p5	CCCATGAAAGCGGTACATGA	ACCACCAAAGCCAAGTACAG	50.0	50.0	58.0	58.0	2124 bp	0.5	59.6