Hotspot

psaA

Genome · 41505-43763 · 2259 bp

Back to Chloroparvula

Pi 0.481

MarkerSeek score 79.60

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: Genome
length_bp: 2259
variable_sites: 931
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	ACACTGGAACTACTGGACGA	CGTGTAGTAGGGTCTTGTGC	50.0	55.0	58.0	58.0	2945 bp	0.5	59.9
psaA_p2	ACACTGGAACTACTGGACGA	TCAAAGTCATGAGCCGTAGC	50.0	50.0	58.0	58.0	2984 bp	0.5	59.8
psaA_p3	ACACTGGAACTACTGGACGA	ATACCGTACCACAAACGTCG	50.0	50.0	58.0	58.0	2963 bp	0.5	59.8
psaA_p4	ACACTGGAACTACTGGACGA	ACCCTGACTAAATCGTGGGA	50.0	50.0	58.0	58.1	2922 bp	0.5	59.5
psaA_p5	CTAGGACATCGCCCTTTCAC	CGTGTAGTAGGGTCTTGTGC	55.0	55.0	58.1	58.0	2677 bp	0.5	59.5