Hotspot

chlB

Genome · 49050-50582 · 1533 bp

Back to Chloroparvula

Pi 0.474

MarkerSeek score 81.10

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 1533
variable_sites: 627
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	GCCCCCAATGTAGGTTCAAT	GGTGGAATGGTAGACACGAC	50.0	55.0	57.8	58.0	2005 bp	0.5	58.7
chlB_p2	GCCCCCAATGTAGGTTCAAT	TCAGTTGGTAGAGCAGTGGA	50.0	50.0	57.8	58.0	1868 bp	0.5	58.6
chlB_p3	GCCCCCAATGTAGGTTCAAT	GAACCTCCGAATGTCTGGAC	50.0	55.0	57.8	58.0	1743 bp	0.5	58.6
chlB_p4	TCAATTCCTACTAGGCCCGT	GGTGGAATGGTAGACACGAC	50.0	55.0	57.8	58.0	1990 bp	0.5	58.6
chlB_p5	TCAATTCCTACTAGGCCCGT	TCAGTTGGTAGAGCAGTGGA	50.0	50.0	57.8	58.0	1853 bp	0.5	58.6