Hotspot

atpF

Genome · 28037-28591 · 555 bp

Back to Chloroparvula

Pi 0.327

MarkerSeek score 82.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: Genome
length_bp: 555
variable_sites: 173
pi: 0.327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GTATTGCTCGTCAGCCAGAA	CTTCGCCCATTAGAACGACA	50.0	50.0	58.0	58.0	1088 bp	0.5	59.9
atpF_p2	GCTAACCCATTCACAGCGTA	CTTCGCCCATTAGAACGACA	50.0	50.0	58.0	58.0	972 bp	0.5	59.8
atpF_p3	GTATTGCTCGTCAGCCAGAA	AGCTGCCTCAATTAGACGTG	50.0	50.0	58.0	58.0	1249 bp	0.5	59.8
atpF_p4	GTATTGCTCGTCAGCCAGAA	CAGCTGCCTCAATTAGACGT	50.0	50.0	58.0	58.0	1250 bp	0.5	59.8
atpF_p5	GTATTGCTCGTCAGCCAGAA	ATAATTCCTGGAGCAGCTGC	50.0	50.0	58.0	58.0	1263 bp	0.5	59.7