Hotspot

accD

Genome · 16017-17180 · 1164 bp

Back to Chloroparvula

Pi 0.278

MarkerSeek score 80.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 1164
variable_sites: 317
pi: 0.278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGTGATCGTCTAGTAGCACG	AATGCGACTGAGTGACATCC	55.0	50.0	58.0	58.0	2574 bp	0.5	59.8
accD_p2	CGTGATCGTCTAGTAGCACG	ATGGAATGCGACTGAGTGAC	55.0	50.0	58.0	58.0	2578 bp	0.5	59.8
accD_p3	CGTGATCGTCTAGTAGCACG	GGAATGCGACTGAGTGACAT	55.0	50.0	58.0	58.0	2576 bp	0.5	59.8
accD_p4	TGCTCAAGGGCGTATTCTTC	AATGCGACTGAGTGACATCC	50.0	50.0	58.0	58.0	2395 bp	0.5	59.7
accD_p5	TGCTCAAGGGCGTATTCTTC	GGAATGCGACTGAGTGACAT	50.0	50.0	58.0	58.0	2397 bp	0.5	59.7