Hotspot

ccsA

SSC · 105666-106634 · 969 bp

Back to Chloris

Pi 0.00654

MarkerSeek score 48.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 9
pi: 0.00654
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1446 bp	1.0	81.8
ccsA_p2	GTGAAATCGGTAGACACGCT	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1447 bp	1.0	81.8
ccsA_p3	TGAAATCGGTAGACACGCTG	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1445 bp	1.0	81.8
ccsA_p4	TGAAATCGGTAGACACGCTG	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1446 bp	1.0	81.8
ccsA_p5	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1450 bp	1.0	81.8