Hotspot

ccsA-ndhD

SSC · 106635-106828 · 194 bp

Back to Chloris

Pi 0.0808

MarkerSeek score 75.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 194
variable_sites: 23
pi: 0.0808
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCATTTGTAGCTTCGA	GTAGAGGCTCTCTTATCCAA	45.0	45.0	57.9	53.3	345 bp	1.0	46.8
ccsA-ndhD_p2	TTCCGCATTTGTAGCTTCGA	AGAGGCTCTCTTATCCAATT	45.0	40.0	57.9	53.1	343 bp	1.0	45.4
ccsA-ndhD_p3	TCCGCATTTGTAGCTTCGAT	AGAGGCTCTCTTATCCAATT	45.0	40.0	57.7	53.1	342 bp	1.0	45.4
ccsA-ndhD_p4	TCCGCATTTGTAGCTTCGAT	GTAGAGGCTCTCTTATCCAA	45.0	45.0	57.7	53.3	344 bp	1.0	45.3
ccsA-ndhD_p5	TTCCGCATTTGTAGCTTCGA	GTAGAGGCTCTCTTATCCAAT	45.0	42.9	57.9	53.9	345 bp	1.0	45.3