Hotspot

rpl33-rps18

LSC · 65688-65947 · 260 bp

Back to Chloris

Pi 0.0385

MarkerSeek score 71.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 15
pi: 0.0385
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	ACGAAAAGGTTGCTTGGATTTT	50.0	36.4	58.0	57.6	442-467 bp	1.0	63.3
rpl33-rps18_p2	CCACAATACACCCGGACAAT	ACGAAAAGGTTGCTTGGATTTT	50.0	36.4	57.9	57.6	435-460 bp	1.0	62.6
rpl33-rps18_p3	AGAATCGCCACAATACACCC	TTACGAAAAGGTTGCTTGGA	50.0	40.0	58.0	55.5	444-469 bp	1.0	62.5
rpl33-rps18_p4	CCACAATACACCCGGACAAT	TTACGAAAAGGTTGCTTGGA	50.0	40.0	57.9	55.5	437-462 bp	1.0	61.8
rpl33-rps18_p5	TTTGTCGTTATTGCCGCAAG	ACGAAAAGGTTGCTTGGATTTT	45.0	36.4	57.7	57.6	400-425 bp	1.0	61.3