Hotspot

petN-trnC-GCA

LSC · 18054-18899 · 846 bp

Back to Chloris

Pi 0.0205

MarkerSeek score 58.80

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 26
pi: 0.0205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTTCTCCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	954-976 bp	1.0	72.8
petN-trnC-GCA_p2	GTCCACTTCTCCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	953-975 bp	1.0	72.8
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	895-917 bp	1.0	72.7
petN-trnC-GCA_p4	AGTCCACTTCTCCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	956-978 bp	1.0	72.3
petN-trnC-GCA_p5	GTCCACTTCTCCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	955-977 bp	1.0	72.3