Hotspot

ycf1

SSC · 111903-117539 · 5637 bp

Back to Chloranthus

Pi 0.0156

MarkerSeek score 51.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 197
pi: 0.0156
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATGTCTTTGTGCACGACA	TCGTCATTGCACAATCTGGT	40.0	45.0	56.5	57.8	175-178 bp	1.0	73.7
ycf1_p2	AATGTCTTTGTGCACGACAA	TCGTCATTGCACAATCTGGT	40.0	45.0	56.5	57.8	174-177 bp	1.0	73.7
ycf1_p3	AAAATGTCTTTGTGCACGACA	TCGTCATTGCACAATCTGGT	38.1	45.0	57.2	57.8	176-179 bp	1.0	71.5
ycf1_p4	AAATGTCTTTGTGCACGACAA	TCGTCATTGCACAATCTGGT	38.1	45.0	57.2	57.8	175-178 bp	1.0	71.5
ycf1_p5	AATGTCTTTGTGCACGACAA	CGTCATTGCACAATCTGGTC	40.0	50.0	56.5	57.5	173-176 bp	1.0	71.0