Hotspot

rpl32-ndhF

SSC · 128279-129442 · 1164 bp

Back to Chloranthus

Pi 0.0307

MarkerSeek score 75.60

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1164
variable_sites: 88
pi: 0.0307
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GTACGTTTCTTTGGAACCGC	CGAAAGGTATGACCCATGCA	50.0	50.0	57.7	58.0	1218-1303 bp	1.0	86.7
rpl32-ndhF_p2	GTACGTTTCTTTGGAACCGC	ACGAAAGGTATGACCCATGC	50.0	50.0	57.7	58.0	1219-1304 bp	1.0	86.7
rpl32-ndhF_p3	GTACGTTTCTTTGGAACCGC	GGAACAAGCAGAAGTCCCAA	50.0	50.0	57.7	58.0	1267-1352 bp	1.0	86.5
rpl32-ndhF_p4	GTACGTTTCTTTGGAACCGC	ACAAGCAGAAGTCCCAATCC	50.0	50.0	57.7	57.8	1264-1349 bp	1.0	85.0
rpl32-ndhF_p5	GTACGTTTCTTTGGAACCGC	CTGGAAGTGGAACGAAAGGT	50.0	50.0	57.7	57.7	1230-1315 bp	1.0	84.9