Hotspot

trnL-UAG-rpl32

SSC · 127179-128125 · 947 bp

Back to Chloranthus

Pi 0.0355
MarkerSeek score 73.80
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
947
variable_sites
76
pi
0.0355
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC ATATCGGGCCGCGATAAAAG 50.0 50.0 58.0 58.3 1057-1085 bp 1.0 85.6
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA ATATCGGGCCGCGATAAAAG 50.0 50.0 58.0 58.3 1058-1086 bp 1.0 85.5
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC GATATCGGGCCGCGATAAAA 50.0 50.0 58.0 58.3 1058-1086 bp 1.0 85.5
trnL-UAG-rpl32_p4 AGCGTGTCTACCGATTTCAC TGGGTACTCTATCCTCGAGC 50.0 55.0 58.0 57.7 1636-1666 bp 0.857143 78.9
trnL-UAG-rpl32_p5 CAGCGTGTCTACCGATTTCA TGGGTACTCTATCCTCGAGC 50.0 55.0 58.0 57.7 1637-1667 bp 0.857143 78.8