Hotspot

ndhD-ccsA

SSC · 125772-126028 · 257 bp

Back to Chloranthus

Pi 0.0341

MarkerSeek score 63.90

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 257
variable_sites: 22
pi: 0.0341
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TCAGTTGACAAGGTGGAAGC	GAGGCATGGGGGTCTTATTG	50.0	55.0	58.0	58.0	536-561 bp	1.0	85.7
ndhD-ccsA_p2	GGATTCTGGACCACGAGAAC	GAGGCATGGGGGTCTTATTG	55.0	55.0	58.0	58.0	627-652 bp	1.0	85.1
ndhD-ccsA_p3	TTCTCGATGGCTTCTCTTGC	GAGGCATGGGGGTCTTATTG	50.0	55.0	58.0	58.0	851-876 bp	1.0	84.0
ndhD-ccsA_p4	ATGGGTTTATTGGTGCTGCA	GAGGCATGGGGGTCTTATTG	45.0	55.0	58.1	58.0	979-1004 bp	1.0	83.4
ndhD-ccsA_p5	GCATATGGGTTGGTTCGGAT	GAGGCATGGGGGTCTTATTG	50.0	55.0	58.0	58.0	1220-1245 bp	1.0	83.1