Hotspot

accD-psaI

LSC · 61408-62081 · 674 bp

Back to Chloranthus

Pi 0.038

MarkerSeek score 70.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 674
variable_sites: 53
pi: 0.038
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGGTTTCTTTCCC	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	822-891 bp	1.0	89.2
accD-psaI_p2	CCACGGTTTCTTTCCCTTGA	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	818-887 bp	1.0	89.2
accD-psaI_p3	TCCACGGTTTCTTTCCCTTG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	819-888 bp	1.0	89.2
accD-psaI_p4	GACAGTACCCGATGGTTCAC	GGTCCCATCGGATCTAGACA	55.0	55.0	58.0	58.0	929-998 bp	1.0	88.9
accD-psaI_p5	GGGCACTTGGAATCCTATGG	GGTCCCATCGGATCTAGACA	55.0	55.0	58.0	58.0	1451-1520 bp	1.0	86.8