Hotspot

trnS-GCU-trnG-UCC

LSC · 8796-9696 · 901 bp

Back to Chloranthus

Pi 0.0453
MarkerSeek score 83.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
901
variable_sites
89
pi
0.0453
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AATCTGCCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.3 55.5 941-1080 bp 1.0 60.9
trnS-GCU-trnG-UCC_p2 CAATCTGCCGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.3 55.5 942-1081 bp 1.0 60.8
trnS-GCU-trnG-UCC_p3 ATCAGAAGGAGCGGAGAGAG ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 1006-1145 bp 1.0 60.0
trnS-GCU-trnG-UCC_p4 GATCAGAAGGAGCGGAGAGA ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 1007-1146 bp 1.0 60.0
trnS-GCU-trnG-UCC_p5 CGGAGAGAGAGGGATTCGAA ACGAATCACACTTTTACCACT 55.0 38.1 58.3 55.5 995-1134 bp 1.0 60.0