Hotspot

rps15-ycf1

SSC · 124600-125027 · 428 bp

Back to Chionographis

Pi 0.0255

MarkerSeek score 68.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 428
variable_sites: 16
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGCGAAGTAAGCCTCTGTATC	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.6	58.3	595-605 bp	1.0	69.7
rps15-ycf1_p2	ATGCGAAGTAAGCCTCTGTA	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.4	58.3	596-606 bp	1.0	68.0
rps15-ycf1_p3	TGCGAAGTAAGCCTCTGTAT	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.4	58.3	595-605 bp	1.0	68.0
rps15-ycf1_p4	GCGAAGTAAGCCTCTGTATCT	CGGCAGTCGTTTCAGTATGT	47.6	50.0	57.3	58.3	594-604 bp	1.0	67.4
rps15-ycf1_p5	ATGCGAAGTAAGCCTCTGTATC	CGGCAGTCGTTTCAGTATGT	45.5	50.0	58.0	58.3	596-606 bp	1.0	65.0