Hotspot

ndhF-rpl32

SSC · 113459-114200 · 742 bp

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Pi 0.0144

MarkerSeek score 54.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 742
variable_sites: 16
pi: 0.0144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AACACATTCCCACCAGTTCC	GGTGGAAACCGATTTAGCCA	50.0	50.0	58.0	58.2	1334-1362 bp	1.0	80.1
ndhF-rpl32_p2	CATTCCCACCAGTTCCCAAA	GGTGGAAACCGATTTAGCCA	50.0	50.0	58.0	58.2	1330-1358 bp	1.0	80.1
ndhF-rpl32_p3	AAAAGCCCACATACGACGAA	GGTGGAAACCGATTTAGCCA	45.0	50.0	57.8	58.2	988-1016 bp	1.0	79.1
ndhF-rpl32_p4	GGGCGTGTAAACCAAAAACC	GGTGGAAACCGATTTAGCCA	50.0	50.0	58.1	58.2	1368-1396 bp	1.0	79.0
ndhF-rpl32_p5	ACCATAATCCCCGCAGTAGT	GGTGGAAACCGATTTAGCCA	50.0	50.0	58.2	58.2	1182-1210 bp	1.0	78.7