Hotspot

rpl14-rpl16

LSC · 81478-81606 · 129 bp

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Pi 0.0271

MarkerSeek score 62.70

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 5
pi: 0.0271
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TTTGAGCTCCGCTGTTATCC	CTGCGTGCAAGATGCCTATA	50.0	50.0	58.0	58.1	222-228 bp	1.0	82.7
rpl14-rpl16_p2	TTTGAGCTCCGCTGTTATCC	CGTGCAAGATGCCTATACGA	50.0	50.0	58.0	57.9	219-225 bp	1.0	82.7
rpl14-rpl16_p3	CCGCTGTTATCCGCTACATT	CTGCGTGCAAGATGCCTATA	50.0	50.0	58.1	58.1	214-220 bp	1.0	82.0
rpl14-rpl16_p4	CCGCTGTTATCCGCTACATT	CGTGCAAGATGCCTATACGA	50.0	50.0	58.1	57.9	211-217 bp	1.0	82.0
rpl14-rpl16_p5	TTTGAGCTCCGCTGTTATCC	TGCGTGCAAGATGCCTATAC	50.0	50.0	58.0	58.4	221-227 bp	1.0	80.4