Hotspot

rpl36-infA

LSC · 80025-80130 · 106 bp

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Pi 0.0314

MarkerSeek score 62.30

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 5
pi: 0.0314
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	CCTACGGATCAGTCGACATT	TCGTTATGATTCAACCCGGG	50.0	50.0	56.8	57.7	227 bp	1.0	69.9
rpl36-infA_p2	GCCTACGGATCAGTCGACAT	TCGTTATGATTCAACCCGGG	55.0	50.0	59.3	57.7	228 bp	1.0	68.6
rpl36-infA_p3	CCTACGGATCAGTCGACATT	CGCAACAAAGATTCGAACGA	50.0	45.0	56.8	57.4	187 bp	1.0	68.3
rpl36-infA_p4	CCTACGGATCAGTCGACATT	TCGCAACAAAGATTCGAACG	50.0	45.0	56.8	57.4	188 bp	1.0	68.3
rpl36-infA_p5	CCTACGGATCAGTCGACATTT	TCGTTATGATTCAACCCGGG	47.6	50.0	57.6	57.7	227 bp	1.0	67.7