Hotspot

petG-trnW-CCA

LSC · 66698-66818 · 121 bp

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Pi 0.0606

MarkerSeek score 79.50

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 11
pi: 0.0606
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGCGGTGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	207-208 bp	1.0	83.7
petG-trnW-CCA_p2	CAATACAGACGCGGTGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	206-207 bp	1.0	83.7
petG-trnW-CCA_p3	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	193-194 bp	1.0	83.6
petG-trnW-CCA_p4	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	229-230 bp	1.0	83.4
petG-trnW-CCA_p5	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	228-229 bp	1.0	83.4