Hotspot

petL-petG

LSC · 66385-66583 · 199 bp

Back to Chionographis

Pi 0.0121

MarkerSeek score 59.50

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 3
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CCGGCTCTATTGGTTGGTTT	GATCACCGCGTCTGTATTGT	50.0	50.0	58.2	58.1	333-341 bp	1.0	80.7
petL-petG_p2	CCGGCTCTATTGGTTGGTTT	TGATCACCGCGTCTGTATTG	50.0	50.0	58.2	58.1	334-342 bp	1.0	80.7
petL-petG_p3	TCAACTATAACCCCGGCTCT	GATCACCGCGTCTGTATTGT	50.0	50.0	57.8	58.1	345-353 bp	1.0	80.5
petL-petG_p4	TCAACTATAACCCCGGCTCT	TGATCACCGCGTCTGTATTG	50.0	50.0	57.8	58.1	346-354 bp	1.0	80.5
petL-petG_p5	ATAACCCCGGCTCTATTGGT	GATCACCGCGTCTGTATTGT	50.0	50.0	58.2	58.1	339-347 bp	1.0	80.5