Hotspot

psbE-petL

LSC · 65086-66288 · 1203 bp

Back to Chionographis

Pi 0.00842

MarkerSeek score 65.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 15
pi: 0.00842
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAACCAACCAATAGAGCCGG	50.0	50.0	57.9	58.2	1373-1381 bp	1.0	80.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGAGCCGGGGTTATAGTTGA	50.0	50.0	57.9	57.8	1361-1369 bp	1.0	80.0
psbE-petL_p3	CAACCCGCAATGAATAGGGA	ACCAATAGAGCCGGGGTTAT	50.0	50.0	57.9	58.2	1367-1375 bp	1.0	79.9
psbE-petL_p4	ACTGACGAATAACCAACCCG	AAACCAACCAATAGAGCCGG	50.0	50.0	57.9	58.2	1386-1394 bp	1.0	79.7
psbE-petL_p5	ACTGACGAATAACCAACCCG	AGAGCCGGGGTTATAGTTGA	50.0	50.0	57.9	57.8	1374-1382 bp	1.0	79.7