Hotspot

ndhC-trnV-UAC

LSC · 51150-51977 · 828 bp

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Pi 0.00644

MarkerSeek score 62.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 8
pi: 0.00644
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AATAGGCGCTAAAACTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	963-1181 bp	1.0	78.6
ndhC-trnV-UAC_p2	GGTCCTTCAGTAATAGGCGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.1	57.8	974-1192 bp	1.0	78.0
ndhC-trnV-UAC_p3	CGGTCCTTCAGTAATAGGCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.9	57.8	975-1193 bp	1.0	77.9
ndhC-trnV-UAC_p4	AATAGGCGCTAAAACTCCGG	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	1045-1263 bp	1.0	76.4
ndhC-trnV-UAC_p5	AATAGGCGCTAAAACTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	962-1180 bp	1.0	75.9