Hotspot

petN-psbM

LSC · 28927-29937 · 1011 bp

Back to Chionographis

Pi 0.00333
MarkerSeek score 62.40
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1011
variable_sites
5
pi
0.00333
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 CCCTTTCACTCGTGGTATGG ACGTAAAAACAGTCAGCCAA 55.0 40.0 58.0 55.8 1078-1093 bp 1.0 64.4
petN-psbM_p2 CCTTTCACTCGTGGTATGGG ACGTAAAAACAGTCAGCCAA 55.0 40.0 58.0 55.8 1077-1092 bp 1.0 64.4
petN-psbM_p3 CTTTCACTCGTGGTATGGGG ACGTAAAAACAGTCAGCCAA 55.0 40.0 58.0 55.8 1076-1091 bp 1.0 64.4
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACAGTCAGCCAA 50.0 40.0 58.2 55.8 1123-1138 bp 1.0 63.3
petN-psbM_p5 CTTTCACTCGTGGTATGGGG TCTACGTAAAAACAGTCAGCCA 55.0 40.9 58.0 57.7 1079-1094 bp 1.0 63.3