Hotspot

rpoB-trnC-GCA

LSC · 26721-27684 · 964 bp

Back to Chionographis

Pi 0.011

MarkerSeek score 61.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 964
variable_sites: 14
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	AAAGGATTTGCAGTCCCCTG	50.0	50.0	58.2	58.1	902-1111 bp	1.0	83.1
rpoB-trnC-GCA_p2	TAAATCCGGGTATTGTGGGC	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.6	58.1	844-1053 bp	1.0	81.8
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	GCCAATTTGTTCATCAGGCG	50.0	50.0	58.2	58.4	945-1154 bp	1.0	80.7
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	AAAGGATTTGCAGTCCCCTG	45.0	50.0	57.4	58.1	903-1112 bp	1.0	79.8
rpoB-trnC-GCA_p5	CAAATTCTTCCGTCAAGCCC	AAAGGATTTGCAGTCCCCTG	50.0	50.0	57.4	58.1	904-1113 bp	1.0	79.4