Hotspot

psbK-psbI

LSC · 6862-7274 · 413 bp

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Pi 0.0265

MarkerSeek score 73.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 413
variable_sites: 16
pi: 0.0265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GCTGCTGCAAGTTTTCGATG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.7	58.0	525-529 bp	1.0	78.6
psbK-psbI_p2	AGCTGCTGCAAGTTTTCGAT	GGATTGCGTCCTGGATCATT	45.0	50.0	58.8	58.0	526-530 bp	1.0	77.8
psbK-psbI_p3	CTTTGTTTGGCAAGCTGCTG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.8	58.0	538-542 bp	1.0	77.5
psbK-psbI_p4	GCTGCTGCAAGTTTTCGATG	TATTCCTCACGTCCGGGATT	50.0	50.0	58.7	58.2	540-544 bp	1.0	76.9
psbK-psbI_p5	GCTGCTGCAAGTTTTCGATG	TTATTCCTCACGTCCGGGAT	50.0	50.0	58.7	58.2	541-545 bp	1.0	76.9