Hotspot

rps16-trnQ-UUG

LSC · 5675-6248 · 574 bp

Back to Chionographis

Pi 0.0108
MarkerSeek score 59.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
574
variable_sites
5
pi
0.0108
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.59
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 665 bp 0.333333 52.5
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 663 bp 0.333333 52.5
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 671 bp 0.333333 52.4
rps16-trnQ-UUG_p4 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 672 bp 0.333333 52.4
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 647 bp 0.333333 51.6