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trnK-UUU-rps16

LSC · 4009-4674 · 666 bp

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Pi 0.0152

MarkerSeek score 69.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 14
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGCACTTAAAAGCCGAGTA	TTTTCTTGAAAACGGCGCTC	50.0	45.0	58.3	57.9	797 bp	0.333333	50.2
trnK-UUU-rps16_p2	CATAGCCGCACTTAAAAGCC	TTTTCTTGAAAACGGCGCTC	50.0	45.0	57.5	57.9	802 bp	0.333333	48.6
trnK-UUU-rps16_p3	CCGCACTTAAAAGCCGAGTA	GCGCTCAACCTACAGTAACT	50.0	50.0	58.3	57.6	783 bp	0.333333	48.1
trnK-UUU-rps16_p4	TGTGAAAGATCATAGCCGCA	TTTTCTTGAAAACGGCGCTC	45.0	45.0	57.3	57.9	812 bp	0.333333	46.9
trnK-UUU-rps16_p5	CATAGCCGCACTTAAAAGCC	GCGCTCAACCTACAGTAACT	50.0	50.0	57.5	57.6	788 bp	0.333333	46.6