Hotspot

rpl32-trnL(UAG)

SSC · 114985-115782 · 798 bp

Back to Chionanthus

Pi 0.0189

MarkerSeek score 67.70

Primer available yes

Label rpl32-trnL(UAG)

Feature Metadata

label_name: rpl32-trnL(UAG)
feature_type: igs
region: SSC
length_bp: 798
variable_sites: 80
pi: 0.0189
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL(UAG)_p1	TGGGACAGCGTTAAAAGCAT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	921-1005 bp	1.0	87.3
rpl32-trnL(UAG)_p2	TGGGACAGCGTTAAAAGCAT	AAAAATAGCACTTGGGCCGA	45.0	45.0	58.1	58.1	1376-1468 bp	1.0	86.5
rpl32-trnL(UAG)_p3	TGGGACAGCGTTAAAAGCAT	GAAGTAGCAAAGCCTTGGGT	45.0	50.0	58.1	58.1	1396-1488 bp	1.0	86.3
rpl32-trnL(UAG)_p4	TGGGACAGCGTTAAAAGCAT	TGAAGTAGCAAAGCCTTGGG	45.0	50.0	58.1	58.1	1397-1489 bp	1.0	86.3
rpl32-trnL(UAG)_p5	TGGGACAGCGTTAAAAGCAT	AACCACTCGGATTGAAGAGC	45.0	50.0	58.1	57.9	1459-1551 bp	1.0	86.2