Hotspot

petD-rpoA

LSC · 80235-80415 · 181 bp

Back to Chionanthus

Pi 0.031

MarkerSeek score 72.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 17
pi: 0.031
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	TGAACATTTGCGCCTAGAAGA	45.0	42.9	57.9	57.9	319-338 bp	1.0	80.6
petD-rpoA_p2	TTTGGTTGGGTATTGGTGCA	TGAACATTTGCGCCTAGAAGA	45.0	42.9	57.9	57.9	320-339 bp	1.0	80.5
petD-rpoA_p3	GTCGCTCTTTGGTTGGGTAT	TGAACATTTGCGCCTAGAAGA	50.0	42.9	57.9	57.9	327-346 bp	1.0	80.2
petD-rpoA_p4	TTGGTTGGGTATTGGTGCAA	TGAACATTTGCGCCTAGAAG	45.0	45.0	57.9	56.4	319-338 bp	1.0	76.5
petD-rpoA_p5	TTGATTGGTACTGCAGTCGC	TGAACATTTGCGCCTAGAAGA	50.0	42.9	58.6	57.9	342-361 bp	1.0	76.4